Result description
Used during early-life immune system assessment (typically ages 1–6), particularly in studies related to gestational exposures, allergies, and immune-mediated disease risk profiling.
- Fresh blood samples are collected and stimulated using TruCulture® tubes with specific ligands (e.g. LPS, zymosan).
- Cytokine concentrations are measured from supernatants.
- Gene expression profiling is performed from cell pellets using RT² Profiler PCR Arrays.
- Specific IgE is quantified in serum using ImmunoCap™ Phadiatop™ Infant test.
The method stimulates innate immune responses in whole blood using standardized ligands. It then analyzes both secreted cytokines and transcriptional changes, providing a high-resolution profile of immune function relevant to early markers of allergy and immune dysregulation.
Addressing target audiences and expressing needs
Our needs are
- Use of research Infrastructure
- Collaboration
- Fellowship to advance my/our research
We specifically need / are looking for
We are also looking for grants and subsidies.
Target audiences
- EU and Member State Policy-makers
- Research and Technology Organisations
- Academia/ Universities
R&D, Technology and Innovation aspects
Current stage description
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Replicability
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Sustainability
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Result submitted to Horizon Results Platform by TARTU ULIKOOL