At The International Research Center in Critical Raw Materials for Advanced Industrial Technologies from the University of Burgos (UBU), we evaluate the human and environmental toxicity of materials applying a broad set of in vitro assays. For the study of human exposure, cell lines representative of different organs (lung, liver, intestine…) are used, together with 3D skin tissue models. For the evaluation of environmental exposures, several microorganisms such as the yeast Saccharomyces cerevisiae, or the bacteria  Vibrio fischeri and Pseudomonas putida are used. The parameters to measure the toxicity include viability, ROS production, irritation, interleukin release, growth curves depending on the organism applied in the tests.